...
2. If you are not satisfied with the results, tweak some of the optional parameters (particularly the local area box size) and re-run beadtrack.
(D) Upscale the Binned .fid File to Full Resolution
1. The final step is to scale the output tracked and binned fiducial file so it is back in the coordinate system of the original, unbinned .preali file:
| Code Block |
|---|
$ imodtrans -i basename.preali basename.bin8.fid basename.fid |
2. You can now proceed with fine alignment in etomo!