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Your reconstruction should already be in the MRC format if it was calculated using IMOD or TxBR. If your reconstruction is from a 4k x 4k camera, you should be able to load the full volume into memory on jane without any performance issues. However, if it is from the 8k x 8k camera or is a montage, you may need to bin it so it will fit into memory. Amira's performance will not suffer.
1. If your volume needs to be binned, use the following command to bin by a factor of 2:
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If you have already binned your volume in the above stepStep 1, run the following command to invert the contrast:
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