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| Code Block |
|---|
$ newstack -scale 255,0 -secs Zmin-Zmax basename_2Dbin2.rec basename_2Dbin2_inverted_trimZ.rec |
If you haven't already binned your volume because it is from a 4k x 4k camera, run the following command to perform the binning and contrast inversion operations simultaneously:
| Code Block |
|---|
$ newstack -bin 2 -scale 255,0 -secs Zmin-Zmax basename.rec basename_2Dbin2_inverted_trimZ.rec |
(C) Loading the Volumes into Amira
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