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3. Repeat step 1 for your volume with inverted contrast (basename_2Dbin2_inverted.rec). You should see a second green button appear in the Pool frame.
(D) Display the Original Reconstruction
1. First, set the background color to solid black. In the upper menu, select View --> Background
1. Right-click on the green button for the original reconstruction in the Pool frame, and select OrthoSlice. You will see an orange OrthoSlice button appear connected to the green button.
Pool --> Open Data --> Select Data --> MRC Volume
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