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Step 2.2 Create the augmented data using CDeep3M:https://github.com/slash-segmentation/Membrane_Myelin_Subtract/blob/main/getMembraneOnly.php

PreprocessTrainingData.py /myelin_images /myelin_labels /myelin_augmented  

Con

Step 2.3  Create the training model:

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runprediction.sh --augspeed 10 --models 1fm  ~/mem_retrain_out ~/em ~/membrane_predictions


Prediction:

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Note that the prediction result contains the myelin layer.


Step 3.2 Run the CDeep3M on the Myelin data:

runprediction.sh --augspeed 10 --models 1fm  ~/myelin_trainednet ~/em ~/myelin_predictions


Step 4 Subtract the myelin layer from the membrane prediction


Convert the membrane result to the binary format:

https://github.com/slash-segmentation/Membrane_Myelin_Subtract/blob/main/convert2Bin.php


Convert the myelin result to the binary format:

https://github.com/slash-segmentation/Membrane_Myelin_Subtract/blob/main/myelin2Bin.php


Invert the myelin binary result:

https://github.com/slash-segmentation/Membrane_Myelin_Subtract/blob/main/invertMyelin.php


Extract the membrane result without myelin:

https://github.com/slash-segmentation/Membrane_Myelin_Subtract/blob/main/getMembraneOnly.php


Create the membrane only overlay:

https://github.com/slash-segmentation/Membrane_Myelin_Subtract/blob/main/runOverlay.php