a) Raw EM data can be found in  /ccdbprod/ccdbprod23/home/CCDB_DATA_USER.portal/CCDB_DATA_USER/acquisition/project_20271/microscopy_5391395/segmentation/testAppOut/em

b) Membrane Prediction folder can be found in /ccdbprod/ccdbprod23/home/CCDB_DATA_USER.portal/CCDB_DATA_USER/acquisition/project_20271/microscopy_5391395/segmentation/testAppOut/predictions-003

c) Myelin Prediction folder can be found in /ccdbprod/ccdbprod23/home/CCDB_DATA_USER.portal/CCDB_DATA_USER/acquisition/project_20271/microscopy_5391395/segmentation/testAppOut/predictions-007

Step 1.0

Find the closest membrane model in the existing models. In this case, we want to use membranes_Aug05_40k, which can be downloaded at https://doi.org/10.7295/W9CDEEP3M50772

Step 1.1 Create the retrain data:

Go to membrane prediction data (a). Create the binary version of the prediction data by using the ImageMagick command:

convert $input.png -threshold 50% $output.png